Primary structure of ribonuclease frombacillus intermedius7P
نویسندگان
چکیده
منابع مشابه
Studies on Ribonuclease S. I. Limited Carboxypeptidase Degradation of Ribonuclease S-protein and Ribonuclease S-peptide: Effects of Changes in Primary Structure on Enzymic Activity.
Specific regions of the polypeptide sequence of pancreatic ribonuclease have been altered by chemical modification and by limited proteolytic digestion in attempts to implicate specific covalent portions of the molecule in the structure and stabilization of the active center. Digestion of the native molecule with trypsin at elevated temperatures has been shown to remove portions of the chain wi...
متن کاملStudies on the Antigenic Structure of Ribonuclease
Alkali-denatured bovine pancreatic ribonuclease (RNase) reacts poorly with antibody to the native molecule, and RNase whose disulfide bridges have been broken by oxidation or reduction is completely inactive with this antiserum. These observations suggest that the antigenicity of the native protein is, in part, attributable to its conformation (1). Oxidized RNase has no detectable immunogenic o...
متن کاملStructure and properties of seminal ribonuclease.
Brand, R. C. & Planta, R. J. (1975) Mol. Biol. Rep. 2, 321-325 Klootwijk, J. & Planta, R. J. (1973) Eur. J. Biochem. 39, 325-333 Maden, B. E. H. & Salim, M. (1974) J. Mol. Biol. 88, 133-164 Maden, B. E. H., Forbes, J., De Jonge, P. & Klootwijk, J. (1975) FEBSLerr. 59,60-63 Perry, R. P. (1976) Annu. Rev. Biochem. 45, 605-629 Planta, R. J., Van den Bos, R. C. & Klootwijk, J. (1972) in Ribosomes: ...
متن کاملThe primary structure of langur (Presbytis entellus) pancreatic ribonuclease: adaptive features in digestive enzymes in mammals.
The primary structure of pancreatic ribonuclease from langur (Presbytis entellus) has been determined. This sequence differs from that of human pancreatic ribonuclease at 14 (11%) of the amino acid positions. Eight of these 14 differences involve changes of charge, with the langur enzyme having five fewer positive charges than the human enzyme. The difference in charge between human and langur ...
متن کاملThe primary structure of porcine pancreatic ribonuclease. I. The distribution and sites of carbohydrate attachment.
Reduced, S-aminoethylated porcine ribonuclease was subjected to tryptic hydrolysis. A sequence of fractionation steps that included gel filtration and chromatography over SE-Sephadex C-50, Dowex 50-X2, and Dowex l-X2 afforded a series of glycopeptide fractions which were recognized to derive from three separate regions of the molecule. Heterogeneity of the attached polysaccharide chains preclud...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1979
ISSN: 0014-5793
DOI: 10.1016/0014-5793(79)80056-3